Chromogranin B (CgB) aka Secretogranin-1, UniProt # P05060, is an acidic member of the granin family found in a variety of neurons and endocrine cells. CgB is a marker in neuroendocrine tumors with plasma levels potentially being associated with disease progression.
Description
|
|
Immunogen
|
HEK293-derived recombinant CgB. Met21-Gly677.
|
Reactivity
|
Human
|
Source
|
Mouse monoclonal IgG1
|
Purification
|
Protein G purified from hybridoma cell culture supernatant
|
Applications
|
ELISA, WB
|
Formulation
|
Lyophilized from PBS with Trelahose
Reconstitute in 100μL ddH20 to 1 mg/mL
|
Shipping
|
Ambient
|
Storage
|
5 years at -20°C to -80°C as supplied
1 month at 4°C after reconstitution with preservative
1 year at -20°C to -80°C after reconstitution
|
Expiration
|
See lot specific CoA
|

|
Human CgB Luminex Assay
Capture: Human CgB monoclonal clone CgB-1E11 (Cat # M2015)
Detection: Human CgB antigen purified polyclonal
Antigen: Recombinant human CgB (Cat # P4007)
|
|
WB detection of native CgB
SDS PAGE 4-12%, reducing conditions
Blocking: 5% NFDM, PBS
Sample: HEK293 whole cell lysate, 76μg
Blot was probed with 4μg/mL M2015 followed by HRP detection antibody. Human CgB was detected at 76kDa.
|