Human Gelsolin (UnitProt # P06396) is a potent calcium regulated actin filament severing protein that works to disassemble and reorganize the actin network. The extracellular isoform, plasma gelsolin, is suggested to act as an actin scavenging protein. Decreased levels of plasma gelsolin have been reported in trauma, burns, rheumatoid arthritis, and alzheimer disease.e.
| Description | |
|---|---|
| Immunogen | Recombinant Human Gelsolin expressed in E. coli |
| Reactivity | Human, Bovine, Swine, Horse, Goat, Canine, Guinea Pig |
| Source | Polyclonal rabbit IgG |
| Purification | Antigen affinity purified |
| Applications | ELISA, WB, IP |
| Formulation | Lyophilized from PBS with trelahose Reconstitute in 100μL ddH20 to 1 mg/mL |
| Shipping | Ambient |
| Shipping | Ambient or cold |
| Storage | 5 years at -20°C to -80°C as supplied 1 month at 4°C after reconstitution with preservative 1 year at -20°C to -80°C after reconstitution |
| Expiration | 06/2022 |
| WB Detection of human gelsolin SDS PAGE 4-12%, reducing conditions Blocking: 5% NFDM, PBS Lane 1: Recombinant human gelsolin (Cat # P4004), 10ng Lane 2: Albumin depleted human plasma, 100μg Lane 3: Albumin depleted human plasma, 10μg Blot was probed with 0.2μg/mL R3005 followed by HRP detection antibody. Human gelsolin was detected at 95kDa. |
