SPARC, Hu mAb (SPC-2F4-%B11)

$285.00

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CAT #: M2020

Secreted protein acidic and rich in cysteine (SPARC), aka osteonectin, UniProt # P09486, is a glyocproetin secreted by a variety of cells and tumors. The biological functions of SPARC vary and include tissue remodeling as well as apoptosis. SPARC is considered a tumor inihbitor in various cancers such as breast, colorectal, pancreatic, and ovarian.

 

Description
Immunogen
E. coli-derived recombinant human SPARC
Reactivity
Human
Source
Mouse monoclonal IgG1
Purification
Protein G purified from hybridoma cell culture supernatant
Applications
ELISA, IP, WB
Formulation
Lyophilized from PBS with Trelahose
Reconstitute in 100μL ddH20 to 1 mg/mL
Shipping
Ambient
Storage
5 years at -20°C to -80°C as supplied
1 month at 4°C after reconstitution with preservative
1 year at -20°C to -80°C after reconstitution
Expiration
See lot specific CoA

Human SPARC Luminex Assay
Capture: Human SPARC monoclonal clone SPC-2F4-5B11 (Cat # M2020)
Detection: Human SPARC monoclonal clone SPC-5H7-2C3 (Cat # M2021)
Antigen: Recombinant human SPARC, E. coli expressed

Immunoprecipitation of native human SPARC
SDS PAGE 4-12%, reducing conditions
Blocking: 5% NFDM, PBS
Sample: Human SPARC purified from human serum with M2020
Blot was probed with 2μg/mL M2020 followed by HRP detection antibody. Human SPARC was detected at 40kDa.