Secreted protein acidic and rich in cysteine (SPARC), aka osteonectin, UniProt # P09486, is a glyocproetin secreted by a variety of cells and tumors. The biological functions of SPARC vary and include tissue remodeling as well as apoptosis. SPARC is considered a tumor inihbitor in various cancers such as breast, colorectal, pancreatic, and ovarian.
| Description | |
|---|---|
| Immunogen | E. coli-derived recombinant human SPARC |
| Reactivity | Human |
| Source | Mouse monoclonal IgG1 |
| Purification | Protein G purified from hybridoma cell culture supernatant |
| Applications | ELISA, IP, does not recognize denatured SPARC |
| Formulation | Lyophilized from PBS with Trelahose Reconstitute in 100μL ddH20 to 1 mg/mL |
| Shipping | Ambient |
| Storage | 5 years at -20°C to -80°C as supplied 1 month at 4°C after reconstitution with preservative 1 year at -20°C to -80°C after reconstitution |
| Expiration | See lot specific CoA |
 | Human SPARC Luminex Assay Capture: Human SPARC monoclonal clone SPC-2F4-5B11 (Cat # M2020) Detection: Human SPARC monoclonal clone SPC-5H7-2C3 (Cat # M2021) Antigen: Recombinant human SPARC, E. coli expressed |
| Immunoprecipitation of native human SPARC SDS PAGE 4-12%, reducing conditions Blocking: 5% NFDM, PBS Sample: Human SPARC purified from human serum with M2021 Blot was probed with 4μg/mL M2020 followed by HRP detection antibody. Human SPARC was detected at 43kDa. |
