Secreted protein acidic and rich in cysteine (SPARC), aka osteonectin, UniProt # P09486, is a glyocproetin secreted by a variety of cells and tumors. The biological functions of SPARC vary and include tissue remodeling as well as apoptosis. SPARC is considered a tumor inihbitor in various cancers such as breast, colorectal, pancreatic, and ovarian.
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Description |
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Immunogen |
E. coli-derived recombinant human SPARC |
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Reactivity |
Human |
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Source |
Mouse monoclonal IgG1 |
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Purification |
Protein G purified from hybridoma cell culture supernatant |
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Applications |
ELISA, IP, WB |
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Formulation |
Lyophilized from PBS with Trelahose Reconstitute in 100μL ddH20 to 1 mg/mL |
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Shipping |
Ambient |
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Storage |
5 years at -20°C to -80°C as supplied 1 month at 4°C after reconstitution with preservative 1 year at -20°C to -80°C after reconstitution |
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Expiration |
See lot specific CoA |
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Human SPARC Luminex Assay Capture: Human SPARC monoclonal clone SPC-2F4-5B11 (Cat # M2020) Detection: Human SPARC monoclonal clone SPC-5H7-2C3 (Cat # M2021) Antigen: Recombinant human SPARC, E. coli expressed |
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Immunoprecipitation of native human SPARC SDS PAGE 4-12%, reducing conditions Blocking: 5% NFDM, PBS Sample: Human SPARC purified from human serum with M2020 Blot was probed with 2μg/mL M2020 followed by HRP detection antibody. Human SPARC was detected at 40kDa. |
